ABSTRACT
OBJECTIVE:To study the compatible rationality of Shaoyao gancao decoction by serum HPLC fingerprint. METHODS:The serum HPLC fingerprints of Paeonia lactiflora,Glycyrrhiza uralensis and Shaoyao gancao decoction were compared to determine the number and quantity change of their component in serum. RESULTS:There were eight components in serum of P. lactiflora,23 components in serum of G. uralensis and 32 components in serum of Shaoyao gancao decoction. Some peak areas of serum samples were obviously increased,such as SGT6,SGT7,SGT10,SGT11,SGT14,SGT15,SGT16,SGT17,SGT18,SGT24,SGT25,SGT26,SGT27,SGT28,SGT29 and SGT32. CONCLUSION:The bioavailability is obviously improved after combination of P. lactiflora with G. uralensis,which shows synergism between them. Serum HPLC fingerprint certify the compatible rationality of combination of P. lactiflora and G. uralensis. Established method is simple,accurate and stable.